File:Connexin43-Modulates-Cell-Polarity-and-Directional-Cell-Migration-by-Regulating-Microtubule-Dynamics-pone.0026379.s004.ogv

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Summary

Two colour TIRF microscopy and time lapse imaging showing a Cx43FL-DsRed plaque being transported to the cell membrane along a microtubule (from bottom to top of movie) in a NIH3T3 cell. Images from this video was used to generate the panels in Figure 8C . Green fluorescence is GFP-tubulin, red fluorescence is Cx43-DsRed. Frames were collected at 2.7 fps, playback is at 10 fps. Scale bar = 5 µm.

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Date/TimeThumbnailDimensionsUserComment
current14:53, 3 January 201711 s, 304 × 464 (899 KB)127.0.0.1 (talk)Two colour TIRF microscopy and time lapse imaging showing a Cx43FL-DsRed plaque being transported to the cell membrane along a microtubule (from bottom to top of movie) in a NIH3T3 cell. Images from this video was used to generate the panels in Figure 8C . Green fluorescence is GFP-tubulin, red fluorescence is Cx43-DsRed. Frames were collected at 2.7 fps, playback is at 10 fps. Scale bar = 5 µm.
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