Elek's test
Elek's test, also known as the immuno diffusion technique, is an in vitro virulence test performed upon Corynebacterium diphtheriae.[1] It is used to test for toxigenicity of C. diphtheriae.
It was characterized in 1949.[2] A filter paper strip impregnated with diphtheria antitoxin is buried just beneath the surface of a special agar plate before the agar hardens. Strains to be tested, known positive and negative toxigenic strains are streaked on the agar's surface in a line across the plate, and at a right angle to the antitoxin paper strip. After 24 hours of incubation at 37 degrees Celsius, plates are examined with transmitted light for the presence of fine precipitin lines at a 45-degree angle to the streaks. The presence of precipitin lines indicates that the strain produced toxin that reacted with the antitoxin.
References
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<references />, or <references group="..." />External links
- Definition of Elek's test with diagrams.
- Medical Microbiology 6th edition by Patrick R. Murray, Ken S. Rosenthal, and Michael A. Pfaller
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