U.S. Route 27 in Florida

This article is about the section of U.S. Route 27 in Florida. For the entire route, see U.S. Route 27.

U.S. Highway 27
Route information
Maintained by FDOT
Length:	496.352 mi (798.801 km)
Existed:	1934 – present
Major junctions
South end:	US 1 in Miami
	US 98 near Sebring US 17 / US 92 in Haines City SR 50 in Clermont SR 40 in Ocala US 41 in High Springs US 19 / US 98 / US 221 in Perry US 90 in Tallahassee
North end:	US 27 / SR 1 towards Bainbridge, GA
Highway system
United States Numbered Highways List Bannered Divided Replaced Florida State Highway System Interstate US SR Former Pre-1945 Toll ← SR 26A US 29 → ← SR 62 SR 64 →

U.S. Route 27 (US 27) in Florida is a north–south United States Highway. It runs 481 miles (774 km) from the South Florida Metropolitan Area northwest to the Tallahassee Metropolitan Statistical Area. Throughout the state, US 27 has been designated the Claude Pepper Memorial Highway by the Florida Legislature. It was named after long-time Florida statesman Claude Pepper, who served in both the U.S. Senate and House of Representatives. Nearly the entire length of US 27 in Florida is a divided highway.

Between Miami and Leesburg, US 27 follows SR 25, between Leesburg and Williston, it follows SR 500, between Williston and High Springs, it follows SR 45, between High Springs and Downtown Tallahassee, it follows SR 20, within Downtown Tallahassee it follows SR 61, and between Tallahassee and the Georgia border, it follows State Road 63 (SR 63).

Concurrencies include State Road 80, between South Bay and Clewiston, SR 78 from Moore Haven to Citrus Center, US 98 between Sebring and West Frostproof, US 441 between Leesburg and Ocala, which also includes a concurrency with US 301 between Belleview and Ocala. Others include US 41 between Williston and High Springs, SR 20 between High Springs and Tallahassee, US 129 in Branford, US 19 between Perry and Capps, and SR 61 in Tallahassee.

Route description

Miami through Central Florida

US 27 begins as North 36th Street in Midtown Miami, heading west from US 1 for 4.4 miles (7.1 km) before turning northwest to pass under the western terminus of the Airport Expressway (State Road 112, SR 112). It then proceeds northwest for five miles (8 km) as South Okeechobee Road, parallel to the Miami Canal, forming the southwest boundary of the city of Hialeah. After an interchange with the Palmetto Expressway (SR 826), it continues northwest as North Okeechobee Road for five miles (8 km) before an interchange with the Homestead Extension of the Florida Turnpike. After another four miles (6 km), the highway curves to the north and, after passing the northern terminus of Krome Avenue (SR 997), crosses into Broward County.

US 27, looking south towards the I-75 interchange

The Lake Placid Tower, on US 27. Similar to the Citrus Tower further north in Clermont, which is also on US 27

In Broward County, the highway passes protected wetlands and heavy duty power lines on the west and the outer reaches of the suburban communities of Pembroke Pines and Weston on the east where it curves to the northwest. US 27 then reaches an interchange with I-75 and Alligator Alley which is an elaborate partial cloverleaf with flyovers from US 27, loop ramps from I-75 and no re-entry to either road. From here, the road is surrounded by Everglades-related wilderness and recreational areas before curving to the north toward South Bay, where it intersects State Road 80 and overlaps the road before curving west along the shores of Lake Okeechobee. The highway skirts the southwestern shore of Lake Okeechobee and then heads west through Lake Harbor and then Clewiston, before making a sharp turn to the north towards Moore Haven, where it crosses the Mamie Langdale Memorial Bridge over the Caloosahatchee Canal and makes another sharp turn to the west. The road then intersects with State Road 78, which it overlaps until reaching Citrus Center, then proceeds in a northerly direction toward the central Florida communities such as Lake Placid, where it intersects State Road 70.

The southern terminus of the concurrency with U.S. Route 98 is also the eastern terminus of State Road 66. From here, the hidden routes are State Road 25 and State Road 700. Shortly after this, US 27-98 runs through a commercial strip area before curving to the west at a wye intersection along the south shore of Lake Jackson in Sebring, where State Road 17 begins. The road heads back to the northwest as it runs along and then away from the edge of the lake. North of here, the road runs west of Lake Sebring, but in Avon Park it runs much closer to the shores of Lake Glenada, where it passes through South Florida Community College territory. From here it passes by Lake Lelia, and Lake Anoka, and just east of Avon Park Executive Airport it intersects State Road 64 and the northern terminus of a segment of State Road 17. SR 64 continues east along part of SR 17 as a bi-county extension northeast into Polk County which runs through Lake Wales Ridge State Forest and terminates at the Avon Park Air Force Range. North of here, US 27 carries hidden state routes (SRs 17, 25 and 700) into Polk County until it reaches Sunray Deli Estates, where SR 17 breaks away again, and runs relatively parallel to US 27 until it reaches Haines City. Meanwhile, after making a reverse curve over a bridge above a CSX Railroad Line that is used by Amtrak's Silver Star and Silver Meteor lines, US 98 breaks away in West Frostproof, taking SR 700 with it. In the opposite direction of this intersection is a continuation of County Road 630. After passing by Warner University, Crooked Lake Park and County Road 640, US 27 becomes slightly less rural as it approaches an un-numbered partial cloverleaf interchange with State Road 60 in Lake Wales. North of this point, US 27 becomes a six-lane highway, and remains that way until reaching State Road 540 in Waverly, Florida, where the road narrows down to four lanes again. However, the widening of US 27 to a six-lane highway continues in Polk County between here and SR 542 in Dundee. North of here, US 27 runs through Lake Hamilton, and curves around the eastern shores of the lake for which the community was named. After passing by a pair of gated communities and crossing over a bridge between Middle Lake Hamilton, and the ironically named larger Little Lake Hamilton, it curves north and intersects SR 544. Taking a turn to the northeast after passing by Lake Henry, US 27 encounters another un-numbered interchange with U.S. Route 17/92 in Haines City, and immediately passes over another CSX Railroad Line that is used by Amtrak's Silver Star and Silver Meteor lines. With the exception of a segment between Hammock Lake and Tower Lake, most of US 17 remains a commercial strip, especially as it enters Davenport, and approaches quarter-cloverleaf interchanges on the southeast and northwest corners of the bridge over Interstate 4 at Exit 55.

Citrus Ridge to Santos

US 27 in Minneola, looking south towards the Citrus Tower

North of I-4, US 27 contains more un-numbered interchanges. The first of which is a trumpet interchange with US 192 (State Road 530) on the Polk-Lake County Line in Citrus Ridge, and shortly afterwards an at-grade intersection with County Road 474 (CR 474), although some maps have indicated another interchange here. Most of the road remains a rural four-lane highway with at-grade intersections. After passing by Lake Louisa State Park and winding around the eastern shores of the lake that the park was named for, US 27 approaches yet another diamond interchange without exit numbers at SR 50 in Clermont. After passing the Florida Citrus Tower, the road enters Minneola along the east side of Lake Minneola, where it crosses a bridge over the South Lake-Lake Minneola Scenic Trail, intersects such roads as County Road Old 50, as well as County Road 561, the latter of which it shares a brief concurrency. After this it briefly runs parallel to the southwest side of Florida's Turnpike, until it veers slightly to the west and approaches a quarter-cloverleaf interchange with SR 19 south of Howey-in-the-Hills, which also includes a southbound interchange with Florida's Turnpike. The northbound Turnpike interchange can be found further northwest. At Okahumpka, US 27 intersects two county roads that are extensions of state roads. The first of which is County Road 48, and the next of which is the northern terminus of County Road 33 before running along the western shore of Lake Harris and eastern shore of Lake Denham, the location of Singletary Park. North of Lake Harris, it enters Leesburg where US 27 has an intersection with State Road 44. US 441 later joins US 27, and as a result, the hidden state routes are officially SR 25-500. SR 500 becomes more prominent however, because in Lady Lake, SR 25 branches away as County Road 25. This once served as the southern terminus of the US 27-441 Alternate Route around Lake Weir into Belleview, but still remains a scenic route.

Golf cart bridge over US 27/US 441

Around the Lake-Marion county border, US 27-441 enters a retirement community known as The Villages. Because of the frequent use of street-legal golf cars in the community, bridges and tunnels for these carts can be found throughout the road. US 27-441 leaves The Villages as it approaches County Road 42, a bi-county east-west scenic route through Central Florida. North of here, it serves as the newly constructed bypass that leads to CR 484, in Summerfield. Within Belleview, the road encounters two somewhat important intersections; County Road 25A, and then State Road 35. SR 35 joins US 27-441 in a wrong-way concurrency with SR 500 as the road curves more to the west, until it reaches the intersection with US 301, where SR 35 turns south, and SR 500 becomes the hidden state road for US 27-301-441 for a few blocks. However SR 25 reunites with the triplex at the former eastern terminus of CR 484, and shares a concurrency with SR 25, and US 27-301-441's new secret route becomes SR 25-500. After that the road curves more to the north again. Near the right of way for the formerly proposed Cross Florida Barge Canal, the median for the road widens, but narrows back down to normal again. Before it does, however, it intersects CR 328, where a police station exists between the median. Supports for a bridge that was intended to run over the canal exist behind the police station. To the west of this intersection is the Santos Trailhead of the Cross Florida Greenway.

Santos through High Springs

US 27 entering Fellowship from the south

Before US 27-301-441 enters Ocala, it veers off to the left at an intersection with County Road Old 441 (Southeast Lake Weir Avenue), a former segment of US 441 that eventually leads to Ocala Union Station. The first major intersection after this is 31st Street. Then it crosses under a railroad bridge before reaching the City Limits and the intersections with the northern terminus of CR 475 and crossing SR 464 (17th Street). US 27-301-441 intersects with SR 200 (becoming the new hidden state road until US 301 reaches US 1-23). The highway reaches the heart of Ocala at the intersection with SR 40 (West Silver Springs Boulevard). After this, the road crosses a railroad bridge west of Ocala Union Station. Five blocks later, it reaches the intersection of SR 492(Northwest 10th Street) only to move in the opposite direction, leaving the US 301-441 overlap and taking SR 500 with it. After retaining its status as an independent US highway, US 27 moves from Northwest Tenth Street to Northwest Blitchton Road and has another interchange with Interstate 75, although this one is a mere diamond interchange, as opposed to the elaborate partial cloverleaf with flyovers in Weston. From here it continues northwest through rural Marion County, much of which is surrounded by horse farms. The few truly significant intersection in this region include County Road 464B in Fellowship and then County Road 326 near Emathla.

The last intersections at the Marion-Levy County border are with CR 316 and Levy-CR 335. Though horse farms are scarce along this segment in Levy County, the surroundings remain rural. CR 316 begins again at an intersection with US 27 in East Williston, and as the road enters the City of Williston, it serves as the western terminus of CR 318, crosses a set of CSX railroad tracks, and loses its status as an independent US route. State Road 500 continues straight ahead along ALT US 27, while US 27 turns north and joins US 41(hidden SR 45), as well as State Road 121, which already ran east for a few blocks along ALT US 27. The conurrency with SR 121 is short-lived though, as it branches away to the northeast. The US 27-41 concurrency is one of the few segments that are only two lanes wide, and it begins to lean to the northwest until briefly shifting straight north before approaching Archer, where it intersects CR 346, then a former Florida Railway and Navigation Company right-of-way, and finally State Road 24. After SR 24, US 27-41 curves back northwest but head straight northward through State Road 26 in Newberry and both remain as such until US 41 reaches High Springs, and joins US 441 towards Lake City and points north.

High Springs to Tallahassee

The Frank Norris Bridge in Branford

US 27 heads west along the unsigned SR 20. which is named First Avenue until it crosses the Santa Fe River and enters Columbia County. From here it continues to run northwest, but briefly turns straight west after entering Fort White just before intersecting State Road 47. The road shifts to the right again and turns northwest before leaving the city, but gradually turns back west as it gets closer to Suwannee County. Before the road crosses the Ichetucknee River at the Columbia-Suwannee County Line, it intersects with Southwest Riverside Avenue, which serves as the western terminus of the O'Leno to Ichetucknee Trail and the eastern terminus of the Suwannee River Greenway, a former ACL Railroad line. Both US 27 and the trail run through Hildreth, the home of the Ichetucknee Springs State Park, and then encounters the east end of a multiplex with U.S. Route 129 (State Road 49) which is also the southern terminus of County Road 49. US 27-129 and the trail enter the hamlet of Wachtokha, but the trail remains straight while the road curves slightly to the northwest. A slight curve to the southwest is where both routes officially enter Branford where US 129 turns north onto Hidden SR 249. The Suwannee River Greenway crosses US 27 between the west end of the US 27-129 multiplex and Frank R. Norris Bridge across the Suwannee River, but it was also once the northern terminus of US ALT 129, which followed US 27 across the river into Lafayette County until it reached State Road 349 in Grady.

US 27 in Mayo near the SR 51 intersection. On the right is the M. Pico Building

The road runs through the rural community of Alton without being noticed, but slows down as it enters Mayo where it intersects State Road 51, the northwest corner of which contains the historic Lafayette County Courthouse. After the intersection of Lafayette Street, the road curves northwest again. Outside the city limits, the road intersects various local roads, the most significant of which is County Road 534. The rest of the way, US 27 runs northwest through more of western rural Lafayette County, but it turns straight west again as it approaches Buckville where it intersects the southern terminus of County Road 53 and northern terminus of County Road 534, both of which were once part of State Road 53. The last intersection in Lafayette County is with County Road 348 in Townsend before US 27 becomes surrounded by swampland as it enters Taylor County.

US 27 at the Aucilla River, which is the Madison/Jefferson County border

Swampland continues to surround US 27 until it comes close to Perry, where a small truck weigh station can be found along the westbound shoulder. The road divides momentarily at the intersection of State Road 30, which has controlled turn lanes in the divider, but then turns back into an undivided two lane road as it curves northwest. At Buckeye Nursery Road, US 27 gets the name East Hampton Springs Avenue. East Hampton Springs curves from northwest to southwest once again, and the straight west at Washington Street. The street name changes from East Hampton Springs Avenue to West Hampton Springs Avenue at US 221(hidden SR 55) but also joins U.S. Truck Route 221. Several blocks later the road intersects Byron Butler Parkway which is the northern terminus of the US 19-98-ALT 27 multiplex. Here, U.S. Alternate Route 27 terminates, U.S. 98 and SR 30 leaves US 19 turning straight ahead west onto West Hampton Springs Avenue, and US 27 joins US 19 becoming a four-lane highway again. US 19-27 also continues to carry US Truck 221 until it reaches County Road 359A (Wright Road), but also continues to take SR 20 with it heading straight northwest through the rest of Taylor County, and then Madison County, where it intersects County Road 150. US 19 breaks away in Capps onto State Road 57 on its way through Monticello, but not before resuming a westward direction at the Madison-Jefferson County Line. In Tallahassee, the road becomes the 1957-built Apalachee Parkway, a major east–west thoroughfare. The parkway has a short expressway section just east of the capitol, then is a busy four-lane surface boulevard with service roads for the next few miles, passing the Governor's Square Mall, Tallahassee Mall and many state office buildings. US 27 runs west along this stretch of road however, and the Apalachee Parkway ends at SR 61(Monroe Street) in front of the Florida State Capitol building. US 27 and SR 20 make a sharp turn north along SR 61, but at the intersection of U.S. Route 90 in Florida, SR 20 turns west while US 27 continues north along SR 61.

North from Tallahassee

State Road 63
Location:	Tallahassee–Georgia state line, near Hinson

After the intersection with US 90, hidden State Road 63 officially begins at the intersection of North Monroe Street and Thomasville Road, where State Road 61 resumes its status as a separate exposed route. After crossing Seventh Avenue, it begins to curve to the northwest avoiding Lake Ella. The first intersection during this curve is County Road 158 (Tharpe Street), and is the beginning of SR 63's alternate street name of Old Quincy Highway.

SR 63 approaches its only suffixed auxiliary route in the form of County Road 63A before encountering a partial cloverleaf interchange with Interstate 10 (Exit 199). North of the interchange, it begins to leave the Tallahassee City Limits. A great deal of this segment of SR 63 consists of RV parks and other facilities serving tourists for the Lake Jackson Mounds Archaeological State Park It also serves as the northeast terminus of County Road 0356. After crossing a bridge that runs over a small portion of Lake Jackson, the road simultaneously intersects the northern terminus of State Road 263 and southern terminus of County Roads 157 and 0361.

The road runs along the west side of Tallahassee Commercial Airport, and makes a reverse curve as it approaches a bridge over the Ochlockonee River, where it crosses the Leon-Gadsden County Line and continues to move northwest. After crossing another bridge, the first major intersection in the county is the eastern terminus of County Road 270. Further north, County Road 159 secretly joins US 27/SR 63 in a triple concurrency, and all three routes approach a CSX Railroad line until they curve northeast before entering the Town of Havana. CR 159 branches off on its own to the northwest before US 27/SR 63 curves straight north as it approaches the intersection with State Road 12 and CR 12A. SR 12 joins the road in a brief concurrency before branching off to the east as CR 12, within the Havana Town Limits. The previously mentioned CSX railroad line runs more or less parallel to the road again. The road and the tracks curve back and forth near each other until the tracks take one final turn to the northwest in Hinson, and SR 63 begins to curve back to the northeast before the intersection with County Road 159A. Shortly after this SR 63 curves further to the northeast as it approaches County Road 12B. Finally in Darsey, SR 63 serves as the northern terminus of County Road 157 and the southern terminus of an extension of Georgia State Route 111, before curving to the northwest again and finally terminating at the Florida-Georgia State Line, where US 27 continues onto Georgia State Route 1.

History

A US 27 shield used in Florida prior to 1993

US 27 first entered Florida in 1934, terminating at Tallahassee. In 1949, it was extended to its current terminus in Miami.

From 1956 until 1993, US 27 signs in Florida featured white numbering on a green shield. The "color-coding" of U.S. Routes by the Florida Department of Transportation was stopped when the state could no longer use Federal funds to replace the signs with anything but the standard black-and-white version; a few green US 27 signs remain.

Major intersections

County	Location	mi	km	Destinations	Notes
Miami-Dade	Miami	0.000	0.000	US 1 (Biscayne Boulevard / SR 5) to I-95 / I-195 – Miami Beach	I-195 exit 2B
		1.138	1.831	US 441 (Northwest 7th Avenue / SR 7)
		1.395	2.245	Northwest 10th Avenue to Express Lanes north
		1.651	2.657	SR 933 (Northwest 12th Avenue)
		2.660	4.281	To SR 112 / I-95 / Northwest 22nd Avenue
		3.171	5.103	SR 9 (Northwest 27th Avenue) to SR 112 / I-95 – Airport
	Hialeah	4.405	7.089	SR 948 west (Northwest 36th Street)
		4.43	7.13	SR 112 east to I-95 – Miami Beach	southbound exit and northbound entrance
		4.735	7.620	SR 953 (LeJeune Road) – Miami International Airport
		6.018	9.685	SR 944 east (Northwest 54th Street / Hialeah Drive) / Curtiss Parkway
		6.785	10.919	SR 823 north (Red Road / West 4th Avenue)	no access from US 27 south
	Hialeah Hialeah Gardens	9.43	15.18	SR 826 – Airport	interchange
	Hialeah Gardens	10.844	17.452	SR 932 east (Northwest 103rd Street)
		12.159	19.568	To I-75 / Hialeah Gardens Boulevard / Northwest 116th Way
		14.489	23.318	Turnpike Extension (SR 821) – Orlando, Homestead	Turnpike exit 35
	​	19.247	30.975	SR 997 south (Krome Avenue) – Homestead, Key West
Broward	Pembroke Pines	23.218	37.366	SR 820 east (Pines Boulevard) to I-75
		24.720	39.783	To SR 822 / I-75 / Sheridan Street
	Southwest Ranches	26.776	43.092	To SR 818 / I-75 / Griffin Road – Everglades Holiday Park
	Weston	32.65	52.55	I-75 (Alligator Alley / SR 93) to SR 84 east – Miami, Fort Lauderdale, Naples	I-75 exit 23
Palm Beach	Okeelanta	69.691	112.157	CR 827
	South Bay	73.528	118.332	SR 80 east (Main Street / West Palm Beach Road) to US 441 / SR 715 – Belle Glade, West Palm Beach	south end of SR 80 overlap
Hendry	​	87.203	140.340	CR 835 south (Evercane Road)
	Clewiston	89.384	143.850	W.C. Owen Avenue (CR 832)
	​	95.958	154.429	CR 720 west
	Whidden Corner	98.377	158.322	SR 80 west – LaBelle, Ft. Myers, Big Cypress Seminole Reservation	north end of SR 80 overlap
Glades	​	102.379	164.763	CR 720 east
	​	103.9	167.2	Daniels Road to River Road	interchange
	Moore Haven	104.2	167.7	3rd Street to North Frontage Road	interchange; southbound exit and northbound entrance
	​	106.129	170.798	SR 78 east – Lakeport, Seminole Reservation, Okeechobee	south end of SR 78 overlap
	​	110.110	177.205	SR 78 west – LaBelle	north end of SR 78 overlap
	Harrisburg	120.599	194.085	SR 29 south – LaBelle, Immokalee
	​	121.677	195.820	CR 733 north (3rd Street)
	Palmdale	122.055	196.428	Broadway Street (CR 733 south)
	​	127.979	205.962	CR 17 north (Detjens Dairy Road)
Highlands	​	130.749	210.420	CR 731 south – Venus
	Bairs Den	140.536	226.171	SR 70 – Arcadia, Okeechobee
	​	144.825	233.073	CR 29 south
	​	144.946	233.268	Old State Road 8
	Lake Placid	146.283	235.420	CR 17 north (South Main Avenue)
		147.043	236.643	CR 17A west (Interlake Boulevard) / CR 621 east – Downtown
	​	148.589	239.131	CR 17 south / CR 621 west (Lake June Road) – Lake June in Winter Scrub State Park
	​	150.296	241.878	CR 17 north
	​	157.342	253.217	US 98 south (SR 700) / SR 66 west – Airport, Zolfo Springs, Sebring International Raceway, Okeechobee, Spring Lake	south end of US 98 / SR 700 overlap
	Sebring	160.097	257.651	CR 17A north (Highlands Avenue) – Fairground, Amtrak, truck route to SR 17
		160.847	258.858	SR 17 north – Sebring Historic District
		162.830	262.049	CR 634 west – Highlands Hammock State Park
		163.596	263.282	CR 634 Alt. west (Flare Road / CR 634 east)
		165.816	266.855	Sebring Parkway (CR 634A east) - Sebring
	Avon Park	171.572	276.118	SR 17 south / SR 64 west (Main Street) – Wauchula, Zolfo Springs, Avon Park Air Force Range, Historical District, Airport
		173.074	278.536	CR 17A south / Stryker Road – Avon Park Lakes, truck route to SR 17 / CR 64
Polk	​	177.968	286.412	SR 17 north (Scenic Highway) / North Avon Park Cut-Off – Frostproof
	​	182.234	293.277	US 98 north (SR 700) / CR 630 east – Ft. Meade, Frostproof, Indian Lake Estates	north end of US 98 / SR 700 overlap
	​	183.995	296.111	CR 630A east – Frostproof, Indian Lake Estates
	​	190.269	306.208	CR 640 east / Alta / Bab Cut-Off Road – Babson Park, Webber International University
	Lake Wales	192.372	309.593	CR 17B east (Hunt Brothers Road)
		194.20	312.53	SR 60 – Vero Beach, Tampa, Lake Kissimmee State Park	interchange
		196.100	315.592	Mountain Lake Cut Off Road North - Bok Tower Gardens
		198.530	319.503	CR 17A east (Chalet Suzanne Road) / Thompson Nursery Road – Historic Chalet Suzanne
	Winter Haven	200.143	322.099	SR 540 west (Cypress Gardens Boulevard) / Waverly Road – Legoland, Winter Haven, Waverly
	Dundee	202.883	326.509	SR 542 west (Dundee Road) – Winter Haven, Dundee
	Lake Hamilton	205.476	330.682	CR 546 east (Kokomo Road)
	Winter Haven	207.432	333.829	SR 544 – Winter Haven, Auburndale, Grenelefe
	Haines City	209.28	336.80	US 17 / US 92 (SR 600) to SR 17 – Downtown Haines City	interchange
		210.327	338.488	CR 17 (Old Polk City Road / Main Street) – Business District
		212.866	342.575	CR 547 east / Sanders Road – Davenport
	​	218.15	351.08	I-4 (SR 400) – Orlando, Tampa, St. Petersburg	I-4 exit 55
	​	219.750	353.653	Deen Still Road / Ronald Reagan Parkway (CR 54 east) - Loughman
Lake	Four Corners	226.19	364.02	US 192 east (SR 530) – Disney World, Kissimmee	interchange
		227.892	366.757	CR 474 west
	Clermont	240.96	387.79	SR 50 – Clermont, Orlando	interchange
	Minneola	242.972	391.026	CR Old 50 east (Washington Street)
		243.536	391.933	Main Avenue	former CR 561 south
		244.029	392.727	CR 561 south (Lake Minneola Shores)	south end of CR 561 overlap; former CR 561A south
	​	246.165	396.164	CR 561 north – Astatula	north end of CR 561 overlap
	Groveland	249.52	401.56	SR 19 – Howey-in-the-Hills, Tavares, Groveland	interchange
		249.689	401.835	Turnpike south (SR 91) – Miami	Turnpike exit 285
	​	252.209	405.891	CR 565 south
	​	253.551	408.051	Turnpike north (SR 91) – Ocala	Turnpike exit 289
	Leesburg	258.824	416.537	CR 48 to Turnpike north – Okahumpka, Howey-in-the-Hills
	​	259.879	418.235	CR 33 south – Mascotte, Dade Battlefield Historic State Park
	Leesburg	260.907	419.889	CR 25A north (Connell Road) – Flat Island Preserve
		262.107	421.820	CR 25A south – Flat Island Preserve
		262.741	422.841	SR 44 (Dixie Avenue / South Street) to I-75 / US 441 south – Wildwood, Eustis
		264.329	425.396	US 441 south (SR 500) – Orlando	south end of US 441 / SR 500 overlap
see US 441 (mile 289.300-320.451)
Marion	Ocala	295.480	475.529	US 301 north / US 441 north (North Pine Avenue / SR 200 / SR 500) / SR 492 east (Bonnie Heath Boulevard) to SR 40 – Gainesville, Silver Springs	north end of US 301 / US 441 / SR 200 / SR 500 overlap
		298.29	480.05	I-75 (SR 93) – Lake City, Tampa	I-75 exit 354
	​	301.680	485.507	CR 225A
	​	304.972	490.805	CR 225 north
	Fellowship	305.476	491.616	CR 464B west
	Blitchton	309.361	497.868	CR 326 – Morriston, Zuber
	​	316.027	508.596	CR 316 east
Levy	​	316.203	508.879	CR 335 north
	​	317.248	510.561	CR 316 (Northeast 30th Street)
	​	318.555	512.665	CR 318 east
	Williston	318.965	513.324	CR 323 south (Southeast 8th Street)
		319.602	514.350	US 27 Alt. north / US 41 south / SR 121 south (Noble Avenue / SR 45 south / SR 500 north) – Dunnellon, Tallahassee	north end of SR 500 overlap; south end of US 41 / SR 121 / SR 45 overlap
see US 41 (mile 375.158-408.641)
Alachua	High Springs	353.085	568.235	SR 20 south (Northeast 1st Avenue) / US 41 north (North Main Street / SR 45) – Alachua, Lake City	north end of US 41 / SR 45 overlap; south end of SR 20 overlap
Columbia	​	356.377	573.533	CR 138 west
	​	359.182	578.047	CR 778 east
	Fort White	362.729	583.756	CR 18 east – Worthington Springs
		362.999	584.190	SR 47 (1st Street) – Trenton, Ichetucknee Springs, Lake City
Suwannee	Hildreth	369.456	594.582	CR 137 north
	​	372.470	599.432	US 129 south (SR 49) / CR 49 north – Bell, Live Oak	south end of US 129 overlap
	Branford	376.544	605.989	US 129 north (Southwest Suwannee Avenue / SR 249) – Live Oak, Jasper	north end of US 129 overlap
Lafayette	​	377.889	608.153	SR 349 south – Old Town
	​	380.720	612.709	CR 405	former SR 354
	​	384.766	619.221	CR 416	former SR 354A
	​	385.435	620.298	CR 411	former SR 251
	​	385.561	620.500	CR 412	former SR 251
	​	386.785	622.470	CR 412	former SR 251
	​	389.321	626.551	CR 354 west / CR 405 – Convict Spring	former SR 354
	Mayo	394.061	634.180	SR 51 (Fletcher Avenue) – Steinhatchee, Live Oak
		394.382	634.696	CR 300	former SR 251A
	​	395.457	636.426	CR 320	former SR 251
	​	397.779	640.163	CR 534 / CR 536	former SR 53
	​	398.874	641.925	CR 292 – Lafayette Blue Springs State Park	former SR 251B
	​	400.632	644.755	CR 251 north / CR 350 south	former SR 251 / SR 350
	Buckville	403.058	648.659	CR 53 north / CR 534 – Day, Dowling Park, Madison	former SR 53
	Townsend	405.873	653.189	CR 348 east	former SR 348
Taylor	​	416.660	670.549	Fenholloway Road	former SR 356
	​	416.864	670.878	San Pedro Road	former SR 356
	​	417.613	672.083	CR 30 west (Foley Road) – Foley
	​	418.838	674.054	Foley Cut Off	former SR 356C
	​	420.331	676.457	Buckeye Nursery Road	former SR 361 north
	​	420.457	676.660	Plantation Road	former SR 361 south
	Perry	421.925	679.022	US 221 (Jefferson Street / SR 55)
		422.350	679.706	US 19 south / US 27 Alt. south / US 98 / US 221 Truck south (Byron Butler Parkway / Hampton Springs Avenue / SR 30) – Cross City, St. Petersburg	south end of US 19 / US 221 Truck overlap
		423.309	681.250	CR 356 (West Julia Street)	former SR 356 west
		423.615	681.742	West Ash Street	former SR 356 east
	​	424.399	683.004	Andrew Reams Road	former SR 356B; southbound access only
	​	425.144	684.203	US 221 Truck north / CR 359A (Wright Road / Slaughter Road)	north end of US 221 Truck overlap
	​	426.672	686.662	CR 361 (Pisgah Road / Harrison Blue Road)
	Eridu	438.588	705.839	CR 14 east (Alton Wentworth Road)
Madison	​	440.159	708.367	CR 150 east (Southwest Overstreet Avenue)
Jefferson	Lamont	445.162	716.419	CR 257A south (South Salt Road)
		445.244	716.551	CR 257B north to I-10 – Aucilla
	Capps	451.558	726.712	US 19 north (Florida Georgia Parkway / SR 57) to I-10 – Monticello	north end of US 19 overlap
	Waukeenah	454.072	730.758	CR 259 (Waukeenah Highway) – Wacissa
	​	458.141	737.306	SR 59 to I-10 – Wacissa, Lloyd
Leon	Chaires Crossroads	464.329	747.265	CR 154 (Chaires Cross Road) / CR 2195 (W.W. Kelley Road) – Chaires, Capitola, Wakulla Correctional Institution
	​	466.679	751.047	CR 2197 south (Williams Road)
	Tallahassee	470.916	757.866	US 319 (Capital Circle Southeast / SR 261) – National Guard Armory, Federal Correctional Institution, Tallahassee International Airport
		472.342	760.161	CR 373 south (Blair Stone Road)
		472.86	760.99	Old St. Augustine Road (CR 2196)	eastbound access only
		473.144	761.451	SR 265 north / CR 265 south (Magnolia Drive)
		474.1	763.0	Franklin Boulevard (CR 1555), Thomasville Road (SR 61 north) via Gadsden Street (CR 1557 north)	interchange; northbound exit and southbound entrance
		474.222	763.186	CR 1559 south (Calhoun Street)
		474.287	763.291	SR 61 south (Monroe Street)	south end of SR 61 overlap
		474.388	763.453	SR 366 west (Jefferson Street)
		474.740	764.020	US 90 (Tennessee Street / SR 10 / SR 20 west) – Florida State University, Quincy, Monticello	north end of SR 20 overlap
		475.126	764.641	SR 61 north (Thomasville Road) – Thomasville	north end of SR 61 overlap; south end of SR 63; no left turn southbound
		476.073	766.165	CR 158 west (Tharpe Street)
		478.17	769.54	I-10 (SR 8) – Lake City, Pensacola	I-10 exit 199
		480.070	772.598	CR 0356 west (Fred George Road) / Crowder Road – Lake Jackson Mounds Archaeological State Park
	Lake Jackson	482.951	777.234	SR 263 south / CR 0361 (Capital Circle Northwest / Old Bainbridge Road) – Tallahassee International Airport
Gadsden	​	486.065	782.246	CR 270 west (Shady Rest Road)
	​	488.750	786.567	CR 159 south (Scotland Road)
	Havana	489.948	788.495	SR 159 north – Quincy
		490.787	789.845	SR 12 west / CR 12A east (9th Avenue) – Quincy
		491.039	790.251	CR 12 east (5th Avenue East)
		492.840	793.149	CR 159A south (Potter Woodbery Road)
	​	493.391	794.036	CR 12B east (Glade Road)
	Darsey	496.216	798.582	CR 157 south (Concord-Bainbridge Road) to SR 111 / Darsey-Calvary Road – Calvary, GA
	​	496.352	798.801	US 27 north / SR 1 north – Attapulgus, Bainbridge	Georgia state line
1.000 mi = 1.609 km; 1.000 km = 0.621 mi       Concurrency terminus

See also

• Florida portal
• U.S. Roads portal
• List of U.S. Highways in Florida

Bannered routes

At least four bannered routes of US 27 have existed in Florida, however only one exists today.

• U.S. Route 27 Alternate – Sebring Southgate to Avon Park; former route
• U.S. Route 27 Alternate – Sunray Deli Estates (north of Avon Park) to Haines City; former route
• U.S. Route 27 Alternate – Lady Lake to Belleview; former route co-signed with Alternate U.S. Route 441.
• U.S. Route 27 Alternate – Williston to Perry

External links

• US 27; Florida in Kodachrome (Robert V. Droz)
• US 27 in Florida (SoutheastRoads.com)
• Historic Saint Augustine Road (Florida Auto Trails)

U.S. Route 27
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