N-Formylmethionine

N-Formylmethionine

Names
IUPAC name (S)-2-Formylamino-4-methylsulfanylbutanoic acid
Other names 2-Formylamino-4-methylsulfanyl-butyric acid; Formylmethionine; N-Formyl(methyl)homocysteine
Identifiers
CAS Number	4289-98-9 Y
Abbreviations	fMet
EC Number	224-322-8
Jmol 3D model	Interactive image
PubChem	911
SMILES CSCCC(NC=O)C(O)=O
Properties
Chemical formula	C6H11NO3S
Molar mass	177.22 g/mol
Vapor pressure	{{{value}}}
Supplementary data page
Structure and properties	Refractive index (n), Dielectric constant (εr), etc.
Thermodynamic data	Phase behaviour solid–liquid–gas
Spectral data	UV, IR, NMR, MS
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Infobox references

N-Formylmethionine (fMet) is a derivative of the amino acid methionine in which a formyl group has been added to the amino group. It is specifically used for initiation of protein synthesis from bacterial and organellar genes, and may be removed post-translationally.

fMet plays a crucial part in the protein synthesis of bacteria, mitochondria and chloroplasts. It is not used in cytosolic protein synthesis of eukaryotes, where eukaryotic nuclear genes are translated. It is also not used by Archaea. In the human body, fMet is recognized by the immune system as foreign material, or as an alarm signal released by damaged cells, and stimulates the body to fight against potential infection.

Function in protein synthesis

fMet is a starting residue in the synthesis of proteins in bacteria, and, consequently, is located at the N-terminus of the growing polypeptide. fMet is delivered to the ribosome (30S) - mRNA complex by a specialized tRNA (tRNA^fMet) which has a 3'-UAC-5' anticodon that is capable of binding with the 5'-AUG-3' start codon located on the mRNA.

fMet is coded by the same codon as methionine, AUG. However, AUG is also the translation initiation codon. When the codon is used for initiation, fMet is used instead of methionine, thereby forming the first amino acid of the nascent peptide chain. When the same codon appears later in the mRNA, normal methionine is used. Many organisms use variations of this basic mechanism.

The addition of the formyl group to methionine is catalyzed by the enzyme methionyl-tRNA formyltransferase. This modification is done after methionine has been loaded onto tRNA^fMet by aminoacyl-tRNA synthetase.

Note that methionine can be loaded either onto tRNA^fMet or tRNA^Met. However, transformylase will catalyze the addition of the formyl group to methionine only if methionine has been loaded onto tRNA^fMet and not onto tRNA^Met.

This methionine is removed from majority of proteins (both host and recombinant) by methionine aminopeptidase (MAP). Before aminopeptidase can remove the N-terminal methionine, it must be deformylated by peptide deformylase.

The mitochondria of eukaryote cells, including those of humans, and the chloroplasts of plant cells also initiate protein synthesis with N-formylmethionine (see N-Formylmethionine-leucyl-phenylalanine).

Relevance to immunology

Because fMet is present in proteins made by bacteria but not in those made by eukaryotes, the immune system might use it to help distinguish self from non-self. Polymorphonuclear cells can bind proteins starting with fMet, and use them to initiate the attraction of circulating blood leukocytes and then stimulate microbiacidal activities such as phagocytosis.

Since fMet is present in proteins made by mitochondria and chloroplasts, more recent theories do not see it as a molecule that the immune system can use to distinguish self from non-self. Instead, fMet-containing oligopeptides and proteins appear to be released by the mitochondria of damaged tissues as well as by damaged bacteria, and can thus qualify as an "alarm" signal, as discussed in the Danger model of immunity. The prototypical fMet-containing oligopeptide is N-Formylmethionine-leucyl-phenylalanine (FMLP) which activates leukocytes and other cell types by binding with these cells' formyl peptide receptor 1 (FPR1) and formyl peptide receptor 2 (FPR2) G protein coupled receptors (see also formyl peptide receptor 3). Acting through these receptors, the fMet-containing oligopeptides and proteins are part of the innate immune system; they function to initiate acute inflammation responses but under other conditions function to inhibit and resolve these responses. fMet-containing oligopeptides and proteins also function in other physiological and pathological responses.

See Also

• N-Formylmethionine-leucyl-phenylalanine
• Formyl peptide receptor 1
• Formyl peptide receptor 2
• Formyl peptide receptor 3

External links

• N-Formylmethionine at the US National Library of Medicine Medical Subject Headings (MeSH)