Bisbenzimide
From Infogalactic: the planetary knowledge core
(Redirected from Hoechst 33342)
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Names | |
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IUPAC name
2’-(4-Ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-1H,3’H-2,5’-bibenzimidazole
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Other names
Hoechst 33342
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Identifiers | |
23491-52-3 | |
ChemSpider | 1420 |
Jmol 3D model | Interactive image |
PubChem | 1464 |
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Properties | |
C27H28N6O | |
Molar mass | 452.56 g·mol−1 |
Vapor pressure | {{{value}}} |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references | |
Bisbenzimide is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several bisbenzimides are used and are collectively called Hoechst stains.
File:Hoechst 33342 Stain - Platynereis dumerilii larvae.jpg
Nuclei of Platynereis dumerilii larvae stained with Hoechst 33342.
Application
Bisbenzimides tend to bind to A-T rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.
References
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- Sigma Aldrich Product Information Page
- Fluorescence Spectra: http://www.fluorophores.tugraz.at/substance/463
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- DNA-binding substances
- Fluorescent dyes
- Benzimidazoles
- Piperazines